The young Blues suffered a fifth defeat of the season as local rivals Padiham proved too strong at the Arbories.
Two early goals propelled Padiham to a victory that lifted them above Clitheroe into third place in the Lancashire FA County Under-21 League.
The Blues finished with 10 men after goalkeeper Murray O’Rouke was sent off for handling outside the penalty area with 20 minutes to go.
But the visitors were already 3-0 down and effectively out of the contest after being second best in a feisty derby clash.
Padiham went ahead inside the first five minutes as Ben Ashton got the first of his two goals, ramming the ball home from close-range following a goalmouth scramble.
A slip from Charlie Lloyd then gave Jack Prince the opportunity to double the hosts’ lead with a lovely chip after 13 minutes.
The Blues re-grouped and enjoyed their best spell just before the break with the lively James Scorah carrying a threat down the left.
But the nearest they came to getting a goal back was an effort from Will Mcurtie which was well saved.
And when Ashton increased Padiham’s lead six minutes into the second half, there was no way back for Dennis Hill’s men.
O’Rouke prevented further damage with a superb double save before getting his marching orders.
And the Blues at least deserved credit for holding out with 10 men as Lloyd, taking over in goal, made one fine save and stand-in defender Ollie Stirzaker completed a remarkable goalline clearance to deny Padiham a fourth goal before the end.
Clitheroe: O’Rouke, Devenney, C Lloyd, Rawcliffe, Lyttle (Dewhurst 75), Clegg (Mason 65), Sullivan, K Lloyd (Wood 65), Mcurtie (Pennington 60), Stirzaker, Scorah.